基于现代学徒制的畜牧兽医人才工匠精神培育研究

现代学徒制背景下培育学生工匠精神,是高职院校培养符合企业需求高水平高素质技术技能型人才的重要方法。文章从现代学徒制背景下工匠精神内涵、工匠精神价值及培育工匠精神的路径等方面,探究现代学徒制视域下畜牧兽医专业群学生工匠精神培育的重要性。     

甘州区规模化羊场主要疫病及防控措施探讨

肉羊养殖逐渐向规模化方向发展,但是口蹄疫、小反刍兽疫、羊布病等疫病防控面临形势愈加严重。需要从羊场设计、加强饲养管理、消毒、驱虫、制定合理免疫程序加强防检疫工作等多方面采取综合防控措施,降低疫病风险,取得最大养殖效益。     

华南花岗岩侵蚀区不同植被类型坡面土壤有机碳分布和团聚体稳定性

植物是影响土壤有机碳含量和土壤团聚体稳定性的重要因素。选取华南典型花岗岩侵蚀区荒草地、桉树林、湿地松林和木荷林4种植被类型径流小区的土壤为研究对象,分析测定不同坡位、不同土层深度的土壤有机碳特性和团聚体稳定性等指标,评价不同植被类型对土壤养分的分布特性以及团聚体稳定性差异,明确花岗岩侵蚀退化区较为理想的生态恢复措施,旨在为合理利用土壤、重建坡面植被和改善土壤结构提供科学依据。结果表明:土壤总有机碳(TOC)、全氮(TN)和溶解性有机碳(DOC)含量随土层加深逐渐降低,而林地小区土壤碳氮比(C/N)则相反,荒草地碳氮元素的坡面变异系数(CV)显著高于其他3种林地,其中桉树林地TOC、TN、DOC和C/N的坡面分布的变异系数较荒草地分别降低40%,56.18%,68.5%和25.81%;湿地松林地TOC、TN、DOC和C/N的坡面分布的变异系数较荒草地分别降低62.73%,33.71%,46.46%,58.06%;木荷林地TOC、TN、DOC和C/N的坡面分布的变异系数较荒草地分别降低41.82%,38.2%,51.18%,48.39%,表明林地较荒草地更有利于土壤碳氮在坡面的均质化和有机质的积累。荒草地和木荷林地0.25 mm粒径以上的团聚体在上、中坡位的质量分数显著高于其他植被类型,而林下植被生物量较高的木荷林地的平均质量直径(MWD)和几何平均直径(GMD)显著高于其他植被类型。其中木荷小区水稳性团聚体平均质量直径(MWD)较荒草地、桉树和湿地松分别高20.10%,19.58%,23.20%;几何平均直径(GMD)较荒草地、桉树和湿地松分别高20.00%,19.54%,22.23%,表明在花岗岩侵蚀区林地空间结构较好的林草模式有利于土壤有机碳的积累和土壤结构的稳定。     

有机物料添加对内蒙古河套灌区碱化土壤可溶性有机碳的影响

通过研究不同有机物料添加对内蒙古河套灌区碱化土壤可溶性有机碳含量变化的影响，寻找合理高效的增碳方式，降低碱化土壤有机碳损失。以内蒙古河套灌区轻度、中度盐碱土为对象进行田间试验，设置生物炭（BC）添加、羊粪（GM）添加，对照（CK）3个处理，对比和分析不同有机物料添加后土壤有机碳（SOC）、可溶性有机碳（DOC）、土壤理化性质的变化。结果表明：（1）与CK相比，轻度碱化土壤BC、GM处理土壤DOC含量分别增加3.28%，20.66%，SOC含量增加5.40%，10.30%，中度碱化土壤BC和GM处理下可溶性碳增加41.32%，74.10%，土壤SOC含量增加60.24%，79.16%；（2）轻度碱化土壤BC和GM处理土壤DOC含量与SOC含量呈负相关，中度碱化土壤BC和GM处理下呈正相关；（3）盐碱土壤SOC、DOC含量主要与土壤pH、电导率的变化有关；BC处理较GM处理相比土壤电导率低约1.93%~29.15%，而GM处理土壤pH、碱化度比BC处理低0.31%~1.53%，7.10%~24.63%。总体来看，有机物料添加后均能使土壤中SOC、DOC含量提高，不同程度地降低土壤碱化程度，且羊粪添加较生物炭略好，因此羊粪添加对河套灌区碱化土壤碳含量的提高比生物炭添加更有效，土壤改良效果更好，土壤理化性质改善更明显。     

Dynamics of nitrogen translocation from mature leaves to new shoots and related gene expression during spring shoots development in tea plants (Camellia sinensis L.)

The contribution of N remobilization is crucial for new shoots growth and quality formation during spring tea shoots development. However, the translocation mechanism of N from source leaves to sink young shoots is not well understood. In the present study, ¹⁵N urea was applied to mature tea leaves one week before bud break to track N remobilization in a field experiment. The dynamic changes in plant ¹⁵N abundance, contents of amino acids, and the expression levels of genes related to N metabolism and translocation were followed during the 18‐d development of new spring shoots until three expanding young leaves. The results showed that during the growth of new shoots the amount of ¹⁵N in the shoots increased, whereas the N_dff (N derived from ¹⁵N‐urea) in mature leaves decreased, showing that the foliar‐applied N in mature leaves was readily exported to new shoots. This process was found to be accompanied by decline of chlorophylls. In the mature leaves, expression CsATG18a and CsSAG12 involved in autophagy was dramatically induced (> 4‐fold) at approximately nine days after the bud breaking. The genes involved in the transformation of amino acids, including primarily CsGDH2, CsGDH4, CsGLT3, CsGS1;3, and CsASN2 were upregulated by > 3‐fold after bud breaking. The expression levels of CsATG8A, CsATG9, CsSAG12, CsGS1;1, CsGDH1, and CsAAP6 correlated negatively with the N_dff in mature leaves, but positively with ¹⁵N amount and total N amount in new shoots, suggesting these genes played important roles in N export from mature leaves. In the new shoots, the expression of most genes showed two defined peaks, one on six days and one on 12 days after bud breaking. The expression of CsGS2, CsASN3, CsGLT1, and CsAAP4 positively correlated with the ¹⁵N amount and total N amount in new shoots. These genes might be involved in the transport and re‐assimilation of N from mature leaves. The overall results demonstrated that the translocation of ¹⁵N from mature leaves to new spring shoots was regulated by the genes involved in autophagy, protein degradation, amino acid transformation and transport.     

Intensive organic vegetable production increases soil organic carbon but with a lower carbon conversion efficiency than integrated management

Intensive vegetable production in greenhouses has rapidly expanded in China since the 1990s and increased to 1.3 million ha of farmland by 2016, which is the highest in the world. We conducted an 11‐year greenhouse vegetable production experiment from 2002 to 2013 to observe soil organic carbon (SOC) dynamics under three management systems, i.e., conventional (CON), integrated (ING), and intensive organic (ORG) farming. Soil samples (0–20 and 20–40 cm depth) were collected in 2002 and 2013 and separated into four particle‐size fractions, i.e., coarse sand (> 250 µm), fine sand (250–53 µm), silt (53–2 µm), and clay (< 2 µm). The SOC contents and δ¹³C values of the whole soil and the four particle‐size fractions were analyzed. After 11 years of vegetable farming, ORG and ING significantly increased SOC stocks (0–20 cm) by 4008 ± 36.6 and 2880 ± 365 kg C ha^?1 y^?1, respectively, 8.1‐ and 5.8‐times that of CON (494 ± 42.6 kg C ha^?1 y^?1). The SOC stock increase in ORG at 20–40 cm depth was 245 ± 66.4 kg C ha^?1 y^?1, significantly higher than in ING (66 ± 13.4 kg C ha^?1 y^?1) and CON (109 ± 44.8 kg C ha^?1 y^?1). Analyses of ¹³C revealed a significant increase in newly produced SOC in both soil layers in ORG. However, the carbon conversion efficiency (CE: increased organic carbon in soil divided by organic carbon input) was lower in ORG (14.4%–21.7%) than in ING (18.2%–27.4%). Among the four particle‐sizes in the 0–20 cm layer, the silt fraction exhibited the largest proportion of increase in SOC content (57.8% and 55.4% of the SOC increase in ORG and ING, respectively). A similar trend was detected in the 20–40 cm soil layer. Over all, intensive organic (ORG) vegetable production increases soil organic carbon but with a lower carbon conversion efficiency than integrated (ING) management.     

Modern wheat cultivars have greater root nitrogen uptake efficiency than old cultivars

The availability of nitrogen (N) contained in crop residues for a following crop may vary with cultivar, depending on root traits and the interaction between roots and soil. We used a pot experiment to investigate the effects of six spring wheat (Triticum aestivum L.) cultivars (three old varieties introduced before mid last century and three modern varieties) and N fertilization on the ability of wheat to acquire N from maize (Zea mays L.) straw added to soil. Wheat was grown in a soil where ¹⁵N‐labeled maize straw had been incorporated with or without N fertilization. Higher grain yield in three modern and one old cultivar was ascribed to preferred allocation of photosynthate to aboveground plant parts and from vegetative organs to grains. Root biomass, root length density and root surface area were all smaller in modern than in old cultivars at both anthesis and maturity. Root mean diameter was generally similar between modern and old cultivars at anthesis but was greater in modern than in old cultivars at maturity. There were cultivar differences in N uptake from incorporated maize straw and the other N sources (soil and fertilizer). However, these differences were not related to variation in the measured root parameters among the six cultivars. At anthesis, total N uptake efficiencies by roots (total N uptake per root weight or root length) were greater in modern than in old cultivars within each fertilization level. At maturity, averaged over fertilization levels, the total N uptake efficiencies by roots were 292?336 mg N g^?1 roots or 3.2?4.0 mg N m^?1 roots for three modern cultivars, in contrast to 132?213 mg N g^?1 roots or 0.93?1.6 mg N m^?1 roots for three old cultivars. Fertilization enhanced the utilization of N from maize straw by all cultivars, but root N uptake efficiencies were less affected. We concluded that modern spring wheat cultivars had higher root N uptake efficiency than old cultivars.     

Application of urease inhibitor improves protein composition and bread‐baking quality of urea fertilized winter wheat

Background: Nitrogen losses is an economic problem for wheat production and a high risk to the environment. Therefore, improved N fertilizer management is a key to increasing the N efficiency and minimizing N losses. To increase N efficiency, enhanced fertilizers such as urea combined with urease inhibitor can be used. Aims: The aim of present study was to evaluate the effects of different N forms on grain storage protein subunits in winter wheat and to examine whether the observed changes correlate with parameters of baking quality. Methods: The investigation was performed over two consecutive years at two locations in Germany. Protein subunits were analyzed by SDS‐PAGE. Results: Protein concentrations were similarly increased after fertilization with ammonium nitrate and urea + urease inhibitor. Analysis of the individual storage protein fractions indicated that both fertilizers specifically enhanced ω‐gliadins and HMW glutenins, but the effect was more pronounced in the ammonium nitrate treatment. Application of urea + urease inhibitor had greater influence on the protein composition and resulted in higher specific baking volume as well as the best fresh keeping ability, in comparison with urea treatment. Conclusion: Considering that the urea + urease inhibitor treatment resulted in almost comparable improvements of NUE and baking quality, with the additional benefit of reduced N losses in combination with easy handling, urea + urease inhibitor can be recommended as a viable alternative to both urea alone and ammonium nitrate treatments. This opens up an opportunity for the reduction of N loss in wheat production when use of urea is preferred.     

冀北地区近50 a马铃薯需水量及水分盈亏时空变化特征

为高效利用水资源,提高农业生产效益,根据联合国粮农组织(FAO)推荐的参考作物蒸散计算方法和相关作物系数法,利用河北省马铃薯主要种植区域(冀北地区)23个地面气象站的资料,计算了冀北地区近50a(1969—2018年)马铃薯生育期内的需水量和缺水量,并分析了马铃薯生育期内降水量、有效降水量、需水量、缺水量变化趋势,以及不同区域不同生育期马铃薯需水量、缺水量的变化特征。结果表明:1)近50 a冀北地区马铃薯生育期内降水量、有效降水量年际变化可分为2个阶段:1969—2003年呈减少趋势,气候倾向率分别为–15.68 mm·(10a)~(–1)、–6.61 mm·(10a)~(–1);而2004—2018年呈显著增加趋势,气候倾向率分别为60.07 mm·(10a)~(–1)、9.68 mm·(10a)~(–1)。近50 a平均降水量、有效降水量分别为356.5 mm和148.6 mm;空间上均呈自西向东逐渐递减的带状特征。2)近50a马铃薯生育期需水量和缺水量年际变化也表现出1969—2003年减少、2004—2018年增多的趋势,且需水量多的年份缺水量也多,近50 a平均需水量和缺水量分别为497.8 mm、349.1 mm;空间分布上均呈自坝上高原向坝下山地增多特点,且需水量大的地区缺水量也多。3)马铃薯块茎膨大期需水量最多,期间也是缺水量最多的时期。研究结果显示1969—2018年冀北地区马铃薯生育期内水资源一直处于严重亏缺状态,在生产中需充分考虑马铃薯需水量对气象要素变化的响应,加强水分管理,确保水资源高效利用。